Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection.
Identifieur interne : 000184 ( Main/Exploration ); précédent : 000183; suivant : 000185Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection.
Auteurs : RBID : pubmed:24141078Abstract
Anti-HER-3 antibody was used for the first time in a disposable immunosensor based on indium tin oxide (ITO) substrate for HER-3 quantification. Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (R(ct)) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the "single frequency impedance" technique was first used for characterization of interaction between HER-3 and anti-HER-3. Eventually, the proposed ITO-based immunosensor was applied to artificial serum samples spiked with HER-3.
DOI: 10.1016/j.ab.2013.10.014
PubMed: 24141078
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Electronic address: msezginturk@nku.edu.tr.</nlm:affiliation><country xml:lang="fr">Turquie</country><wicri:regionArea>Division of Biochemistry, Department of Chemistry, Faculty of Science, Namık Kemal University, Tekirdağ</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="RBID">pubmed:24141078</idno><idno type="pmid">24141078</idno><idno type="doi">10.1016/j.ab.2013.10.014</idno><idno type="wicri:Area/Main/Corpus">000351</idno><idno type="wicri:Area/Main/Curation">000351</idno><idno type="wicri:Area/Main/Exploration">000184</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Anti-HER-3 antibody was used for the first time in a disposable immunosensor based on indium tin oxide (ITO) substrate for HER-3 quantification. Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (R(ct)) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the "single frequency impedance" technique was first used for characterization of interaction between HER-3 and anti-HER-3. Eventually, the proposed ITO-based immunosensor was applied to artificial serum samples spiked with HER-3.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">24141078</PMID><DateCreated><Year>2014</Year><Month>01</Month><Day>13</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0309</ISSN><JournalIssue CitedMedium="Internet"><Volume>446</Volume><PubDate><Year>2014</Year><Month>Feb</Month><Day>1</Day></PubDate></JournalIssue><Title>Analytical biochemistry</Title><ISOAbbreviation>Anal. Biochem.</ISOAbbreviation></Journal><ArticleTitle>Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection.</ArticleTitle><Pagination><MedlinePgn>9-18</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ab.2013.10.014</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0003-2697(13)00494-6</ELocationID><Abstract><AbstractText>Anti-HER-3 antibody was used for the first time in a disposable immunosensor based on indium tin oxide (ITO) substrate for HER-3 quantification. Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (R(ct)) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the "single frequency impedance" technique was first used for characterization of interaction between HER-3 and anti-HER-3. Eventually, the proposed ITO-based immunosensor was applied to artificial serum samples spiked with HER-3.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Canbaz</LastName><ForeName>Mehmet Çetin</ForeName><Initials>MÇ</Initials><Affiliation>Division of Biochemistry, Department of Chemistry, Faculty of Science, Namık Kemal University, Tekirdağ, Turkey.</Affiliation></Author><Author ValidYN="Y"><LastName>Sezgintürk</LastName><ForeName>Mustafa Kemal</ForeName><Initials>MK</Initials><Affiliation>Division of Biochemistry, Department of Chemistry, Faculty of Science, Namık Kemal University, Tekirdağ, Turkey. Electronic address: msezginturk@nku.edu.tr.</Affiliation></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>10</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Biochem</MedlineTA><NlmUniqueID>0370535</NlmUniqueID><ISSNLinking>0003-2697</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biosensor</Keyword><Keyword MajorTopicYN="N">Cancer biomarkers</Keyword><Keyword MajorTopicYN="N">HER-3</Keyword><Keyword MajorTopicYN="N">ITO</Keyword><Keyword MajorTopicYN="N">Single frequency impedance</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>8</Month><Day>7</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>10</Month><Day>7</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>10</Month><Day>9</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>10</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24141078</ArticleId><ArticleId IdType="pii">S0003-2697(13)00494-6</ArticleId><ArticleId IdType="doi">10.1016/j.ab.2013.10.014</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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